| pKa and weak acid assignment |
| Determining the pKa and concentration ratio of a protien in solution. |
|
This assignment utilizes a protein that acts as a weak acid,
according to the equation:
HProtein + H2O <==> Protein- + H3O+ Ka(Protein) 1. (2 pts) The cabinet named "Protein Solutions" contains a solution labeled "Protein". This solution contains a dilute mixture of the protein and HCl. What is Ka of the protein? (Please show your work.) 2. (3pts) Based on you answer to part 1, at what pH will the ratio [Protein-]/[HProtein] = 0.75? (Please show your work.) 3. (5pts) Create 100ml of solution such that when you add between 1 and 5ml of the protein solution of part 1, the ratio [Protein-]/[HProtein] = 0.75. Please test that your solution works. Also please describe what you did, and report the solutions and amounts you mixed together to make your 100ml solution. |