Enzyme Activity Lab

Observations:

Part A: The Effect of Temperature on Enzyme Activity

Test Tube	
   Contents
	
Volume of Oxygen Produced	
Interpretations	

1	
1mL of liver
+ cold water(4◦ C)
+ 5 ml H2O2			

2

1mL of liver
+ cold water(21◦ C)
+ 5 ml H2O2			

3	
1mL of liver
+ warm water(37◦ C)
+ 5 ml H2O2			

4	
1mL of liver
+ warm water(51◦ C)
+ 5 ml H2O2			

Part B:  The Effect of pH on Enzyme Activity

Test Tube	
Contents
	
Volume of Oxygen Produced	
Interpretations	

6	
1mL of liver
+ buffer at pH 4
+ 5 ml H2O2			

7	
1mL of liver
+ buffer at pH 7
+ 5 ml H2O2			

8 	
1mL of liver
+ buffer at pH 10
+ 5 ml H2O2